Search

This monograph focuses on modern femtosecond laser microscopes for two photon imaging and nanoprocessing, on laser tweezers for cell micromanipulation as well as on fluorescence lifetime imaging (FLIM) in Life Sciences. The book starts with an introduction by Dr. Wolfgang Kaiser, pioneer of nonlinear optics and ends with the chapter on clinical multiphoton tomography, the novel high resolution imaging technique. It includes a foreword by the nonlinear microscopy expert Dr. Colin Sheppard. Contents Part I: Basics Brief history of fluorescence lifetime imaging The long journey to the laser and its use for nonlinear optics Advanced TCSPC-FLIM techniques Ultrafast lasers in biophotonics Part II: Modern nonlinear microscopy of live cells STED microscopy: exploring fluorescence lifetime gradients for super-resolution at reduced illumination intensities Principles and applications of temporal-focusing wide-field two-photon microscopy FLIM-FRET microscopy TCSPC FLIM and PLIM for metabolic imaging and oxygen sensing Laser tweezers are sources of two-photon effects Metabolic shifts in cell proliferation and differentiation Femtosecond laser nanoprocessing Cryomultiphoton imaging Part III: Nonlinear tissue imaging Multiphoton Tomography (MPT) Clinical multimodal CARS imaging In vivo multiphoton microscopy of human skin Two-photon microscopy and fluorescence lifetime imaging of the cornea Multiscale correlative imaging of the brain Revealing interaction of dyes and nanomaterials by multiphoton imaging Multiphoton FLIM in cosmetic clinical research Multiphoton microscopy and fluorescence lifetime imaging for resection guidance in malignant glioma surgery Non-invasive single-photon and multi-photon imaging of stem cells and cancer cells in mouse models Bedside assessment of multiphoton tomography

About the Book: This book `Advanced Mathematics` is primarily designed for B.Tech., IV Semester (EE and EC branch) students of Rajasthan Technical University. The subject matter is discussed in a lucid manner. The discussion is covered in five units: Unit I: deals with Numerical Analysis, Unit-II: gives different aspects of Numerical Analysis, Unit-III: Special Function, Unit-IV:Statistics and Probability, Calculus of Variation and Transforms are discussed in Unit V. All the theoretical concepts are explained through solved examples. Besides, a large number of unsolved problems on each top.

This book presents a systematic approach to the experimental, theoretical, and numerical investigation of reinforced concrete (RC) T-beams strengthened in shear with glass-fibre-reinforced polymers (GFRP) with variation in transverse steel reinforcements. It discusses experiments conducted on simply supported RC T-beams for control beams with and without transverse steel reinforcements and beams strengthened in shear with GFRP sheets and strips in different configurations, orientations, and variation of layers for each type of stirrup spacing. The book also includes a detailed numerical study using ANSYS performed in two stages. The first stage consists of selecting and testing relevant materials in the laboratory to establish the physical and mechanical properties of the materials. The second stage then involves testing beams for shear under two-point static loading systems. The test results demonstrate the advantage of using an externally applied, epoxy-bonded GFRP sheets and strips to increase the shear capacity of the beams. The finite element method (FEM) analysis results verify the experimental results. The book will serve as a valuable resource for researchers and practicing civil engineers alike.

An interdisciplinary, easy-to-understand introduction, covering fundamental theory and practical applications. Featuring numerous operational examples, and interpretation of radar observations, this is a perfect resource for scientists and engineers working on or with radars, as well as senior undergraduate and graduate students.

An effort has been made to investigate the topic using terminology that is as straightforward as possible in order to make it more simply digestible for pupils. The book has a number of illustrations, such as flowcharts and diagrams that make it simple for students to comprehend complex ideas. It is the author's honest desire that both students and academicians would take something helpful away from reading this book. I am hoping that both the students and the teachers will have positive reactions to this book. We are open to hearing recommendations regarding any and all aspects of the profession. We take full responsibility for any deviations or errors that may have been overlooked, and we would be extremely appreciative if readers would bring them to our attention if they did occur.

“Advanced Research Methodology: Innovative Approaches and Applications” is a comprehensive guide designed to address the evolving needs of researchers in various disciplines. This book bridges the gap between traditional methodologies and cutting-edge practices, offering a well-rounded perspective on advanced research techniques. The text covers a wide array of topics, including experimental and non-experimental designs, data collection methods, and data analysis techniques. Special emphasis is placed on the integration of technology and innovative tools that enhance research quality and efficiency. Each chapter is meticulously crafted to provide not only theoretical foundations but also practical applications that researchers can implement in their work. Furthermore, this book highlights the importance of ethical considerations, collaboration, and adaptability in research practices. By examining real-world case studies and emerging trends, readers will gain insights into how to effectively address contemporary challenges in research methodology. Ultimately, this book aims to empower researchers with the skills and knowledge necessary to conduct high-quality, impactful research in an increasingly complex world.

This book provides a collection of in-depth reviews broadly related to the mechanisms of viral replication as applied to various viruses of critical relevance for human or animal disease. Specifically, this book contains six different chapters: 1) Influenza A Virus Multiplication and the Cellular SUMOylation System; 2) West Nile Virus: Basic Principles, Replication Mechanism, Immune Response and Important Genetic Determinants of Virulence; 3) Hepatitis B Virus Genetic Diversity: Disease Pathogenesis; 4) An Overview of the Immune Evasion Strategies Adopted by Different Viruses With Special Reference to Classical Swine Fever Virus; 5) Viral Replication Strategies: Manipulation of ER Stress Response Pathways and Promotion of IRES-Dependent Translation; 6) Antiviral Replication Agents. This book is aimed at students, scholars, professors, and investigators who are peripherally related to, or somehow intrigued by, the different areas of virology covered in this book, as well as at those individuals with greater expertise and knowledge in the topics herein presented who may want up-to-date in depth reviews related to such topics.