An Investigation Into Glucose Tran[s]porter Proteins in Fish
This research aimed to identify glucose transporter proteins (GLUTs) or GLUT-like isoforms in Pacific hagfish and Atlantic hagfish heart tissue. Amino acid and nucleotide comparisons of existing sugar transporter proteins were used in polymerase chain reaction (PCR) primer and probe design. Pacific hagfish (Eptatretus stouti) and Atlantic hagfish (Myxine glutinosa) tissues (heart, liver, intestine), as well as rainbow trout (Oncorhynchus mykiss), sea lamprey (Petromyzon marinus), spiny dogfish (Squalus acanthias), tilapia (Tilapia nilotica and Tilapia zilli), striped bass (Morone saxatilis), and white sturgeon (Acipenser transmontana) heart tissues were investigated. Reverse transcriptase-PCR (RT-PCR) experiments identified gene products resembling general transport genes in Atlantic hagfish and rainbow trout tissues. Northern hybridization experiments identified possible GLUT or other transporter transcripts in heart tissues from Pacific hagfish (5̃.0 kb), sea lamprey (600 bp, 1.5 kb), rainbow trout (980 bp, 1.35 kb, 1.9 kb), striped bass (2.0 kb), and white sturgeon (1.8 to 5.0 kb). A definitive GLUT or GLUT-like isoform was not identified in any hagfish or fish species studied.