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The evolution of immunological research; Preparation of antigens; Physicochemical characterization of antigen preparations; Antibodies; The agglutination test; The precipitation test; The immunodiffusion test; The flocculation test; Hemagglutination tests; The lysis assays; Complement and properdin assays; The complement fixation test; Immune inhibition assay; Immune neutralization tests; Immunohistochemical tests; Immunophysical assays; Assays for incomplete antibodies and antigens; Immune cellulotropic and histotropic reactions.