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This book offers low-cost and rapid molecular assays for the characterization of mutant plant germplasm. Detailed protocols are provided for the desiccation of plant tissues; the extraction of high-quality DNA for downstream applications; the extraction of single-strand-specific nucleases for single nucleotide polymorphism; and small insertion/deletion discovery using standard agarose gel electrophoresis. The methods described can be applied in any laboratory equipped for basic molecular biology and do away with the need for expensive freezers and toxic organic compounds. With the appropriate validation of sample quality and longevity, they can provide sufficient DNA for a variety of molecular applications, such as marker studies and TILLING, at approximately one tenth of the cost per sample when compared to commercial kits.

This fascinating study explores how our prehistoric ancestors developed rituals from everyday life and domestic activities. Richard Bradley contends that for much of the prehistoric period, ritual was not a distinct sphere of activity. Rather it was the way in which different features of the domestic world were played out until they took on qualities of theatrical performance. With extensive illustrated case-studies, this book examines farming, craft production and the occupation of houses, all of which were ritualized in prehistoric Europe. Successive chapters discuss the ways in which ritual has been studied, drawing on a series of examples that range from Greece to Norway and from Romania to Portugal. They consider practices that extend from the Mesolithic period to the Early Middle Ages and discuss the ways in which ritual and domestic life were intertwined.