The Estimation of Sample Size Required in Chemical Limnology and Autecology of Shelled Invertebrates
Calculation of sample size is based on the central limit theorem for a normal distribution with mean zero, variance unity and the tail value equal to alpha. The confidence coefficient is 1 - 2alpha. In the field of chemical limnology and autecology of shelled invertebrates, most chemical parameters must be transformed to obtain a normal distribution. The exception is pH, which is already transformed. Some parameters, such as carbonate and carbon dioxide, have an exponential distribution because of the carbonate-bicarbonate fence at pH 8.3. The sample size is a function of the parameter measured, primarily variance, mean and range.